Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq.

TitleSingle-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq.
Publication TypeJournal Article
Year of Publication2016
AuthorsRedmond D, Poran A, Elemento O
JournalGenome Med
Volume8
Issue1
Pagination80
Date Published2016 07 27
ISSN1756-994X
KeywordsAdaptive Immunity, Animals, Gene Expression Regulation, Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Humans, Jurkat Cells, Mice, Receptors, Antigen, T-Cell, alpha-beta, RNA, Messenger, Sequence Analysis, RNA, Single-Cell Analysis, T-Lymphocytes, V(D)J Recombination
Abstract

Accurate characterization of the repertoire of the T-cell receptor (TCR) alpha and beta chains is critical to understanding adaptive immunity. Such characterization has many applications across such fields as vaccine development and response, clone-tracking in cancer, and immunotherapy. Here we present a new methodology called single-cell TCRseq (scTCRseq) for the identification and assembly of full-length rearranged V(D)J T-cell receptor sequences from paired-end single-cell RNA sequencing reads. The method allows accurate identification of the V(D)J rearrangements for each individual T-cell and has the novel ability to recover paired alpha and beta segments. Source code is available at https://github.com/ElementoLab/scTCRseq .

DOI10.1186/s13073-016-0335-7
Alternate JournalGenome Med
PubMed ID27460926
PubMed Central IDPMC4962388
Grant ListR01 CA194547 / CA / NCI NIH HHS / United States
T32 GM083937 / GM / NIGMS NIH HHS / United States