Submitted by als2076 on August 12, 2020 - 10:18am
Title | DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Kasap C, Elemento O, Kapoor TM |
Journal | Nat Chem Biol |
Volume | 10 |
Issue | 8 |
Pagination | 626-8 |
Date Published | 2014 Aug |
ISSN | 1552-4469 |
Keywords | Antineoplastic Agents, Benzamides, Cell Line, Tumor, Clustered Regularly Interspaced Short Palindromic Repeats, Drug Resistance, Neoplasm, Endonucleases, Epigenesis, Genetic, Genome, High-Throughput Nucleotide Sequencing, Humans, Imidazoles, Kinesin, Molecular Targeted Therapy, Mutation, Naphthoquinones, Quinazolines |
Abstract | To identify physiological targets of drugs and bioactive small molecules, we developed an approach, named DrugTargetSeqR, which combines high-throughput sequencing, computational mutation discovery and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-based genome editing. We applied this approach to ispinesib and YM155, drugs that have undergone clinical trials as anticancer agents, and uncovered mechanisms of action and identified genetic and epigenetic mechanisms likely to cause drug resistance in human cancer cells. |
DOI | 10.1038/nchembio.1551 |
Alternate Journal | Nat. Chem. Biol. |
PubMed ID | 24929528 |
PubMed Central ID | PMC4123312 |
Grant List | R01 GM098579 / GM / NIGMS NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States GM98579 / GM / NIGMS NIH HHS / United States T32GM007739 / GM / NIGMS NIH HHS / United States |