Title | Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Meyer KD, Saletore Y, Zumbo P, Elemento O, Mason CE, Jaffrey SR |
Journal | Cell |
Volume | 149 |
Issue | 7 |
Pagination | 1635-46 |
Date Published | 2012 Jun 22 |
ISSN | 1097-4172 |
Keywords | 3' Untranslated Regions, Adenosine, Codon, Terminator, Methylation, RNA Processing, Post-Transcriptional, RNA, Messenger, RNA, Untranslated, Transcriptome |
Abstract | Methylation of the N(6) position of adenosine (m(6)A) is a posttranscriptional modification of RNA with poorly understood prevalence and physiological relevance. The recent discovery that FTO, an obesity risk gene, encodes an m(6)A demethylase implicates m(6)A as an important regulator of physiological processes. Here, we present a method for transcriptome-wide m(6)A localization, which combines m(6)A-specific methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq). We use this method to identify mRNAs of 7,676 mammalian genes that contain m(6)A, indicating that m(6)A is a common base modification of mRNA. The m(6)A modification exhibits tissue-specific regulation and is markedly increased throughout brain development. We find that m(6)A sites are enriched near stop codons and in 3' UTRs, and we uncover an association between m(6)A residues and microRNA-binding sites within 3' UTRs. These findings provide a resource for identifying transcripts that are substrates for adenosine methylation and reveal insights into the epigenetic regulation of the mammalian transcriptome. |
DOI | 10.1016/j.cell.2012.05.003 |
Alternate Journal | Cell |
PubMed ID | 22608085 |
PubMed Central ID | PMC3383396 |
Grant List | F32 MH095353 / MH / NIMH NIH HHS / United States T32 CA062948 / CA / NCI NIH HHS / United States T32CA062948 / CA / NCI NIH HHS / United States 1F32MH095353-01 / MH / NIMH NIH HHS / United States 1R01NS076465-01 / NS / NINDS NIH HHS / United States MH080420 / MH / NIMH NIH HHS / United States R01 NS076465 / NS / NINDS NIH HHS / United States R01 MH080420 / MH / NIMH NIH HHS / United States |