Submitted by als2076 on August 12, 2020 - 10:18am
Title | Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Redmond D, Poran A, Elemento O |
Journal | Genome Med |
Volume | 8 |
Issue | 1 |
Pagination | 80 |
Date Published | 2016 07 27 |
ISSN | 1756-994X |
Keywords | Adaptive Immunity, Animals, Gene Expression Regulation, Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Humans, Jurkat Cells, Mice, Receptors, Antigen, T-Cell, alpha-beta, RNA, Messenger, Sequence Analysis, RNA, Single-Cell Analysis, T-Lymphocytes, V(D)J Recombination |
Abstract | Accurate characterization of the repertoire of the T-cell receptor (TCR) alpha and beta chains is critical to understanding adaptive immunity. Such characterization has many applications across such fields as vaccine development and response, clone-tracking in cancer, and immunotherapy. Here we present a new methodology called single-cell TCRseq (scTCRseq) for the identification and assembly of full-length rearranged V(D)J T-cell receptor sequences from paired-end single-cell RNA sequencing reads. The method allows accurate identification of the V(D)J rearrangements for each individual T-cell and has the novel ability to recover paired alpha and beta segments. Source code is available at https://github.com/ElementoLab/scTCRseq . |
DOI | 10.1186/s13073-016-0335-7 |
Alternate Journal | Genome Med |
PubMed ID | 27460926 |
PubMed Central ID | PMC4962388 |
Grant List | R01 CA194547 / CA / NCI NIH HHS / United States T32 GM083937 / GM / NIGMS NIH HHS / United States |